C. elegans, one of the most attractive and popular genetic model organism, is used to address questions and advance our understanding of several complex biological processes. The course will provide a general practical introduction to using C. elegans in research. It will cover the pros and cons of using C. elegans compared to other model organisms. The course participants will have hands on experience with popular techniques such as RNAi, genetic crosses, transgenic and GFP reporter strains. Thus, during the course the participants will not only be familiarized with using C. elegans as model organism but also be able to interact and network with experts in the field. The practical part of this course is complemented by lectures given by several internationally renowned C. elegans researchers.
The experiments will include the studies of the life cycle and early development of wild type worms and the phenotypic analysis of mutants. It will cover:
- Maintenance, decontamination and synchronization of worms
- Growth, development and basic anatomical traits
- Classical genetic experiments including crosses and mapping experiments
- Mutagenesis and gene-knockdown experiments using RNAi.
- Generation of transgenic worms.
- Molecular and cell biology techniques including single-worm PCR, antibody staining and visualization.
- Introduction to the most useful web resources and databases for worm scientists.
- Design of CRISPR strategies
- Ben Lehner, CRG, Barcelona
- Julián Cerón Madrigal, IDIBELL, Barcelona
- Germano Cecere, Pasteur Institute, France
- Luis Cachella, IMP, Austria
- Peter Meister, University of Bern, Switzerland
- Nick Stroustrup, CRG, Barcelona
- Marcos Perez, CRG, Barcelona
- Eric Cornes Maragliano, Institut Pasteur, Paris
- Karinna Rubio Peña, IDIBELL, Barcelona
- Jennifer Semple, Univeristy of Bern, Switzerland
- Xènia Serrat Farran, IDIBELL, Barcelona
This course is targeting junior researchers (Technicians, Masters and PhD students, Postdocs, PIs) with interests in the C.elegans field.
Duration:Starting on Sunday September 10th at 3 pm and finishing on Saturday September 16th at 4 pm.
Number of participants: 12
Registration fee: 700 Euros for academic researchers and two spots reserved for CRG Alumni with 20% discount (includes participation in workshop, all the necessary tools to follow the course, didactic material, coffee breaks, lunches and welcome dinner).
Only online registration is accepted
Registration Closes: 15th May 2017
Participants will be communicated by the end of May 2017 if they have been selected for the course.
CRG – Centre for Genomic Regulation