Courses@CRG: Tissue Engineering Course: From Stem Cells to Organoids
Organoids are revolutionizing the fields of developmental and stem cell biology as they have enabled the in vitro generation of complex structures resembling whole organs. They can be generated either from resident progenitors in adult organs or from pluripotent stem cells (ES and iPSc). When stem cells are grown in a 3D environment, they recapitulate the mechanisms of organogenesis seen in vivo. They are able to differentiate, go through the processes of cell-sorting and acquire the specific tissue patterning to develop into several endoderm- mesodem and ectoderm-derived tissues, mimicking their in vivo counterparts. These complex structures provide a unique opportunity to model human organ development in a system remarkably similar to development in vivo.
Selected participants will get an overview of the background and current methods for the generation and characterization of pluripotent stem cells, primordial germ cells, organoids and their genetic manipulation by CRISPR/Cas9 technology. They will have the opportunity to learn and practice how to generate and characterize:
- Mouse embryonic stem cells and induced pluripotent stem cells by means of morula aggregation and teratoma assays
- Primordial germ cells
- Cerebral, liver and intestinal organoids
- Genetically modified organoids by CRISPR/Cas9 technology
Structure of the course
This intensive 8 day hands on course will be composed of talks from renowned international experts in the fields and the sessions will include both theoretical background and hands on practice. Each day will start with a theoretical overview of the particular subfield. This will be followed by a more in depth theoretical session directly linked to the practical session in the afternoon. Practical sessions are going to take place in the CRG training facilities, consisting of fully equipped wet and cell culture labs. At the end of each day the analysis and evaluation of the results will be performed in the bioinformatics teaching laboratory.
- Laura Batlle (CRG, Barcelona, Spain)
- Bernhard Payer (CRG, Barcelona, Spain)
Speakers and instructors
- Meritxell Huch (University of Cambridge, UK)
- Jennifer Nichols (University of Cambridge, UK)
- Thomas Graf (CRG, Barcelona, Spain)
- Luigi Aloia (University of Cambridge, UK)
- Amanda Andersson Rolf (University of Cambridge, UK)
- Marisa Karow (University of Munich, Germany)
- Martin Gigirey (CRG, Barcelona, Spain)
- Tian Tian (CRG, Barcelona, Spain)
- Hannah Benisty (CRG, Barcelona, Spain)
- Jim Swoger (CRG, Barcelona, Spain)
- Carlo Carolis (CRG, Barcelona, Spain)
- Pauline Audergon (CRG, Barcelona, Spain)
- Antonio Tarruell (CRG, Barcelona, Spain)
- Jacqueline Severino (CRG, Barcelona, Spain)
7 days: Starting on the 28th of July 2017 at 9 am and finishing the 4th of August 2017 at 5 pm.
Number of participants: 16
Registration fee: 1000 Euros for academic researchers, 2000 Euros for industry (includes participation in workshop, all the necessary tools to follow the course, didactic material, coffee breaks, lunches, networking event).
Only online registration is accepted.
Registration Closes: 15th April 2017
Worldwide participants will be selected according to their CV and motivation letter submitted.
Selected Participants will be notified via email by the end of April 2017.
CRG – Centre for Genomic Regulation
CRG - Training