Postdoc - The role of AR in stromal regulation of prostate cancer
Your function within the department
Prostate cancer is the most frequently diagnosed cancer in men, and represents the second cause of male cancer-related death. Prostate cancer relies on signaling through the androgen receptor (AR) for tumor maintenance and progression. Androgen-deprivation therapy remains a cornerstone of treatment for advanced prostate cancer. However, castration resistance will inevitably develop in all patients, with high morbidity and mortality as hallmarks. It is commonly appreciated that the tumor microenvironment plays a decisive role in prostate cancer progression. The microenvironment is composed of multiple resident and recruited cells, including immune cells. There is convincing evidence from preclinical and clinical studies that immune cells like lymphocytes and neutrophils play an important role in determination of the fate of the tumor. Not only epithelial cancer cells, but also these immune cells express AR. Preclinical studies have shown that AR in lymphocytes and neutrophils is functional and regulates immune cell polarization. However, consequences of AR signaling in immune cells for cancer development remain underexplored and elusive. We intend to investigate the functional significance of AR signalling in lymphocytes and neutrophils in the human prostate cancer microenvironment. Lymphocytes and neutrophils will be isolated from prostate cancer samples and in vitro models of these immune cells will be applied. Moreover, also the role of AR signalling in prostate cancer cells in attraction of immune cells will be evaluated. Tecniques used will include Immuno histochemistry, FACS sorting, co-culturing, CHiP-seq, RNA-seq and bioinformatics analyses.
The project will be carried out at the laboratory of Molecular Genetics and Molecular Pathology of the Netherlands Cancer Institute (NKI-AVL) in Amsterdam. Project leaders are dr. André M. Bergman and dr. Wilbert Zwart. The NKI-AVL harbours a modern and rich infrastructure in a highly international and collaborative research environment where world-class fundamental and translational research is being conducted.
We are looking for a motivated postdoc with a strong background in molecular biology, genomics and/or immunology and with affinity for, histology, FACS, genomics and next-generation sequencing technologies.
Your career opportunities and terms of employment
You will work in a dynamic international scientific environment and collaborate with colleagues at Antoni van Leeuwenhoek Hospital, the NKI and at other institutes. Postdocs at Antoni van Leeuwenhoek can participate in our new postdoc career development program that focusses on personal development. Does this job description appeal to you and can you identify yourself with the following conditions then we would like to invite you for an interview.
The temporary employment will be for a period up to 4 years. The gross salary per month will be from € 3.363,- to € 3.774,- according to the FWG-function group 55 and depends on previous experience. The terms of employment will be in accordance with the CAO Ziekenhuizen (Collective Labour Agreement for Hospitals).
Want more information?
For more information, please contact dr. A.M. Bergman, Oncologist, tel. +31-20-5122569 or dr. W. Zwart, Group leader, tel: +31-20-5122101