Courses@CRG: CRISPR-CAS9 tool: From Gene to Function
The CRISPR-Cas9 technology, which is revolutionizing research that relies on editing genes, enables researchers to construct novel experiments in a wide range of fields—from basic biomedical research to biotechnology and medicine. It is now possible to create knock-ins, knock-outs and mutations of any gene in any cell line or organism with high efficiency.
This hands on course will provide an overview of tools for the experimental design of proteins, genome editing and cell engineering using the CRISPR-Cas9 system and hands-on training on using bioinformatics tools, generation of knock-in /knock-out, and target validation using the most current technologies.
Expert scientists in the field will hold round table discussions with the participants.
The participants will gain knowledge in:
- Advanced investigation methods in enzymology and protein science and become familiar with the latest discoveries and the underlying principles, methods and potential applications of the CRISPR/Cas9 system.
- Design of target specific CRISPR/Cas9 reagents for a specific genomic target and be able to analyze and assess the results obtained using the CRISPR/Cas9 system.
- Selection of appropriate delivery vectors and editing strategies for a given project.
- Generation of gene knock-out or knock-in modified cells.
- Methods for mutation detection and cell manipulation using the most current technologies.
- Carlo Carolis, Centre for Genomic Regulation (CRG), Barcelona, Spain
- Luciano Di Croce, CRG, Barcelona, Spain
Confirmed speakers and instructors:
- Marc Güell, Wyss Institute, Harvard University, USA
- Rory Johnson, University of Bern, Switzerland
- Mihail Sarov, (Max Planck Institute, Germany)
- Laura Batlle, CRG, Spain
- Katrina Broadbent, CRG, Spain
- Valerio Di Carlo, CRG, Spain
- Martin Gigirey, CRG, Spain
- Julien Villeneuve, CRG, Spain
- Gurpreet Balrey, Merck
Registration fee: 500 Euros for academic researchers and 1000 Euros for industry. (includes participation in the workshop, a detailed course guide containing all lecture materials, laboratory protocols, coffee breaks, lunches and welcome dinner). Two spots are reserved for CRG Alumni with a 20% discount.
Number of participants: 16
Worldwide participants will be selected according to their CV and Motivation Letter.
Duration: Starting on Monday the10th of October 2017 at 9am and finishing on Friday the 16th of October 2017 at 5pm.
Only online registration is accepted
Registration closes: 15th June 2017
Participants will be contacted by the end of June 2017 if they have been selected for the course.
CRG Training Unit
Centre for Genomic Regulation (CRG)