EU-LIFE Postdoc Exchange Seminar: Extending the tools for gene expression regulation and exploring epitranscriptomic translation regulation

On November 4, from 10-11 AM (CET), James Bryson will present his work in a Postdoc Exchange Seminar. 
James is a postdoctoral researcher at BRIC, visiting the group of Eva Novoa at the CRG to present his work. James' visit is supported by the EU-LIFE Postdoctoral Exchange Programme.  

The title of his seminar is: Extending the tools for gene expression regulation and exploring epitranscriptomic translation regulation

Abstract

Tools for gene expression regulation have rapidly expanded beyond the traditional Cas9. More recent generations of CRISPR variants offer invaluable properties for robust and multiplexed regulation of gene expression. Here the development of Cas12 and Cas13 derived CRISPR tools are discussed alongside a computational tool for facilitating the targeting crRNA design and assembly. We are subsequently employing Cas12 to systematically screen the influence of small nucleolar RNAs, responsible for laying modifications on ribosomal RNA, to assess for impacts on cell fate. Extending this exploration of epitranscriptomic influences on translational regulation, I will touch on a method I have been developing looking to directly pair epitranscriptomic information between ribosomal RNA and mRNA to assess how the two interact with one another.

For EU-LIFE members only, if you'd like to attend please reach out to contact@eu-life.eu.